Search results for "research paper"

showing 10 items of 477 documents

Floral scent in a sexually deceptive Ophrys orchid: from headspace collections to solvent extractions

2018

Sexually deceptive orchid flowers use visual, tactile and olfactory cues of female insects in order to attract males of one or a few closely related species as pollinators. Ophrys L. is the most species-rich genus of sexually deceptive orchids in the Mediterranean Basin. Despite Ophrys pollinated by Andrena male bees use alkanes and mainly alkenes with specific double-bond positions as key signals that trigger pseudocopulatory behavior, some volatile organic compounds (VOCs) with low molecular weight were found as long-range attractants non-eliciting copulatory behavior. Since floral scents in Ophrys have been extensively studied by solvent extractions here we aimed to understand which flor…

0106 biological sciences0301 basic medicineAndrenapollinationPollinationAndrenaPlant ScienceFlowers01 natural sciencesGas Chromatography-Mass Spectrometry03 medical and health sciencesPollinatorvolatile organic compoundsBotanyOphrys panormitanaOrchidaceaeOphrysOrchidaceaebiologySettore CHIM/06 - Chimica Organicabiology.organism_classificationPseudocopulation030104 developmental biologyOdorantsSettore BIO/03 - Botanica Ambientale E ApplicataKovats retention indexGas chromatography010606 plant biology & botanyResearch Paper
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Potential risk evaluation for unintended entry of genetically modified plant Propagating material in Europe through import of seeds and animal feed –…

2019

Significant attention has been drawn to the adventitious and technically unavoidable presence of genetically modified (GM) organisms in the food and feed imported into the European Union (EU), while the potential presence of GM seeds in material for cultivation is less studied. Here we report a study from an EU member state, Latvia, during years 2017–2018 regarding monitoring for the presence of GM seeds in certified seed and animal feed material. Eighty-two and 28 samples of seeds intended for cultivation were analyzed in 2017 and 2018, respectively. One soybean sample contained MON40-3-2 soybean seeds (0.09 ± 0.01%) and one maize sample contained MON810 maize seeds (0.08 ± 0.01%). In addi…

0106 biological sciences0301 basic medicineAnimal feedFood Genetically ModifiedFood ContaminationGerminationGenetically modified cropsBiology01 natural sciencesZea mays03 medical and health sciencesmedia_common.cataloged_instanceAnimalsEuropean UnionEuropean unionmedia_commonbusiness.industryPotential riskPlants Genetically ModifiedAnimal FeedLatviaGenetically modified organismBiotechnology030104 developmental biologySeedsSoybeansbusinessAgronomy and Crop Science010606 plant biology & botanyFood ScienceBiotechnologyResearch Paper
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Mycorrhizal symbiosis primes the accumulation of antiherbivore compounds and enhances herbivore mortality in tomato

2021

Abstract Plant association with arbuscular mycorrhizal fungi (AMF) can increase their ability to overcome multiple stresses, but their impact on plant interactions with herbivorous insects is controversial. Here we show higher mortality of the leaf-chewer Spodoptera exigua when fed on tomato plants colonized by the AMF Funneliformis mosseae, evidencing mycorrhiza-induced resistance. In search of the underlying mechanisms, an untargeted metabolomic analysis through ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS) was performed. The results showed that mycorrhizal symbiosis had a very limited impact on the leaf metabolome in the absence of stress, but significantly m…

0106 biological sciences0301 basic medicineAzelaic acidPhysiologyPlant Science01 natural sciences03 medical and health sciencesMetabolomicsSolanum lycopersicumSymbiosisTandem Mass SpectrometrySpodoptera exiguaMycorrhizaeBotanyExiguamedicineMetabolomeAnimalsMetabolomicsmycorrhiza induced resistanceHerbivoryArbuscular mycorrhizaSymbiosisHerbivorebiologyAcademicSubjects/SCI01210AlkaloidfungiFungifood and beveragesbiology.organism_classificationResearch PapersArbuscular mycorrhizaspodoptera exigua030104 developmental biologyDefence primingPlant—Environment InteractionsMycorrhiza induced resistance Spodoptera exiguaChromatography Liquid010606 plant biology & botanymedicine.drug
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C4-like photosynthesis and the effects of leaf senescence on C4-like physiology in Sesuvium sesuvioides (Aizoaceae).

2019

Sesuvium sesuvioides represents a young C4 lineage with C4-like metabolism: CO2 compensation points range between C4 and C3–C4 intermediate values, and Rubisco was detected in bundle sheath and mesophyll.

0106 biological sciences0301 basic medicineC4 photosynthesisPhysiologyPlant SciencePhotosynthetic efficiencyPhotosynthesis01 natural sciencesCarbon Cycle03 medical and health sciencesportulacelloid leaf anatomyBotanyC4-likePhotosynthesisC4 photosynthesisbiologyRuBisCOVascular bundlebiology.organism_classificationResearch PapersEnzyme assayCarbonPlant Leaves030104 developmental biologySesuviumAizoaceaebiology.proteinAizoaceaecarbon isotope valuesimmunolocalization of Rubisco and PEPCMesophyll Cells010606 plant biology & botanyPhotosynthesis and MetabolismJournal of experimental botany
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Two maize Kip-related proteins differentially interact with, inhibit and are phosphorylated by cyclin D–cyclin-dependent kinase complexes

2017

Highlight Maize Kip-related proteins can be differentially phosphorylated by different cyclin D–cyclin-dependent kinase complexes and this influences their performance as cyclin-dependent kinase inhibitors.

0106 biological sciences0301 basic medicineCDKsPhysiologyCyclin DPlant Developmental BiologyPlant ScienceZea mays01 natural scienceslaw.inventionCyclins D03 medical and health sciencesGene Expression Regulation PlantCyclin-dependent kinaselawCyclin DPhosphorylationKinase activityKinase inhibitionCyclin-Dependent Kinase Inhibitor ProteinsPlant ProteinsbiologyKinaseKRP phosphorylationfood and beveragesICK/KRPsCyclin-Dependent Kinases030104 developmental biologyZea mays.Biochemistrybiology.proteinCyclin-dependent kinase complexRecombinant DNAPhosphorylationResearch Paper010606 plant biology & botanyCyclin-dependent kinase inhibitor proteinJournal of Experimental Botany
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Responsiveness of metallothionein and hemocyanin genes to cadmium and copper exposure in the garden snail Cornu aspersum.

2020

Abstract Terrestrial gastropods express metal‐selective metallothioneins (MTs) by which they handle metal ions such as Zn2+, Cd2+, and Cu+/Cu2+ through separate metabolic pathways. At the same time, they depend on the availability of sufficient amounts of Cu as an essential constituent of their respiratory protein, hemocyanin (Hc). It was, therefore, suggested that in snails Cu‐dependent MT and Hc pathways might be metabolically connected. In fact, the Cu‐specific snail MT (CuMT) is exclusively expressed in rhogocytes, a particular molluscan cell type present in the hemocoel and connective tissues. Snail rhogocytes are also the sites of Hc synthesis. In the present study, possible interacti…

0106 biological sciences0301 basic medicineDNA ComplementaryPhysiologymedicine.medical_treatmentSnailsGastropodaSnailBiology010603 evolutionary biology01 natural sciences03 medical and health sciencesstressbiology.animalGastropodaparasitic diseasesGeneticsmedicineMetallothioneinAnimalsMolecular BiologyEcology Evolution Behavior and SystematicsMetal metabolismBase Sequencefungimetal metabolismMidgutHemocyaninbiology.organism_classificationResearch PapersRespiratory protein030104 developmental biologybioaccumulationBiochemistryGene Expression RegulationMetalsHemocyaninsAnimal Science and ZoologyMetallothioneinCornu aspersumCopperrespirationCadmiumResearch PaperJournal of experimental zoology. Part A, Ecological and integrative physiology
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Molecular phylogeny and forms of photosynthesis in tribe Salsoleae (Chenopodiaceae).

2016

Evolution of C3–C4 intermediate and C4 lineages are resolved in Salsoleae (Chenopodiaceae), and a model for structural and biochemical changes for the evolution of the Salsoloid form of C4 is considered.

0106 biological sciences0301 basic medicineRecurrent evolutionPhysiologyBlotting WesternPlant ScienceChenopodiaceaewestern blotsPhotosynthesis01 natural sciences03 medical and health sciencesMicroscopy Electron TransmissionBotanyPhotosynthesisChenopodiaceaeCladePhylogenyCarbon IsotopesbiologyPhylogenetic treeC2 pathway15. Life on landCarbon Dioxidebiology.organism_classificationGlycine Dehydrogenase (Decarboxylating)CO2 compensation pointPhenotypePlant Leaves030104 developmental biologyCompensation pointC3–C4 intermediatesMolecular phylogeneticsTEMleaf anatomyAncestral character state reconstruction010606 plant biology & botanyResearch PaperJournal of experimental botany
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C3cotyledons are followed by C4leaves: intra-individual transcriptome analysis ofSalsola soda(Chenopodiaceae)

2016

The genome of Salsola soda allows a transition from C3 to C4 photosynthesis. A developmental transcriptome series revealed novel genes showing expression patterns similar to those encoding C4 proteins.

0106 biological sciences0301 basic medicineSalsolaC4 photosynthesisfood.ingredientSalsolaPhysiologyPlant ScienceChenopodiaceaecotyledonBiology01 natural sciences03 medical and health sciencesfoodRNA seqBotanyPhotosynthesisChenopodiaceaedevelopmentSalsola sodaCarbon IsotopesleafCaryophyllalesGene Expression Profilingfood and beveragesbiology.organism_classificationCaryophyllalesPlant Leaves030104 developmental biologyMRNA SequencingSeedlingTranscriptomePhosphoenolpyruvate carboxylaseCotyledonResearch Paper010606 plant biology & botanyJournal of Experimental Botany
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Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.

2017

Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusively identified. Thus, very little is known about the function of Trxs in this cellular compartment. Previously, we studied the intracellular localization of PsTrxo1 and confirmed its presence in mitochondria and, interestingly, in the nucleus under standard growth conditions. In investigating the nuclear function of PsTrxo1 we identified proliferati…

0106 biological sciences0301 basic medicineTFs transcription factorsOverexpressionBiologíaBiFC bimolecular fluorescence complementationClinical BiochemistryCell Culture TechniquesTobacco BY-2 cells01 natural sciencesBiochemistryTBY-2 tobacco bright yellow-2DTT 14-dithiothreitolBimolecular fluorescence complementationThioredoxinsGene Expression Regulation PlantTrx thioredoxinlcsh:QH301-705.5GFP green fluorescent proteinlcsh:R5-920biologyProliferating cell nuclear antigen (PCNA)Cell cycleGlutathione3. Good healthCell biologyMitochondriaNTR NADPH thioredoxin reductaseProtein TransportDEM diethyl maleateRT-qPCR Reverse transcription quantitative polymerase chain reactionThioredoxinlcsh:Medicine (General)Oxidation-ReductionAMS 4-acetamido-4-maleimidylstilbene-22-disulfonic acidResearch PaperPCNA proliferating cell nuclear antigenOex overexpressingCell cycleNucleusThioredoxin o103 medical and health sciencesROS reactive oxygen speciesDownregulation and upregulationProliferating Cell Nuclear AntigenTobaccoDAPI 46-diamidine-2-phenylindolmCBM monochlorobimaneCellular compartmentCell NucleusCell growthOrganic ChemistryBotánicaPeasMolecular biologyYFP yellow fluorescent proteinProliferating cell nuclear antigenTBS Tris-buffered salineOD optical density030104 developmental biologylcsh:Biology (General)Cell cultureRNA reactive nitrogen speciesbiology.proteinPrx peroxiredoxinBSA bovine serum albumin010606 plant biology & botanyRedox biology
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An ancestral allele of grapevine transcription factor MYB14 promotes plant defence

2016

Highlight The molecular mechanisms underlying the elevated inducibility of stilbene in pathogen-resistant Vitis sylvestris can be explained by the increased inducibility of the MYB14 promoter.

0106 biological sciences0301 basic medicinestilbene synthaseGenotypePhysiologyMYB14Ultraviolet Raysflg22Plant ScienceResveratrol01 natural sciencesModels Biological03 medical and health scienceschemistry.chemical_compoundPlasmopara viticolaOnium CompoundsGenotypePlant ImmunityVitisJasmonateAllelePromoter Regions GeneticTranscription factorAllelesPlant Proteinschemistry.chemical_classificationGeneticsUV.biologyPhytoalexinfungifood and beveragesbiology.organism_classificationgrapevine (V. sylvestris)030104 developmental biologychemistryOomycetesPlasmopara viticolaSalicylic acid010606 plant biology & botanyResearch PaperTranscription FactorsJournal of Experimental Botany
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